England’s Hannah Botterman and May Campbell will play no part in the Women’s Six Nations as they require surgery.
Prop Botterman will have a second operation on an ankle injury sustained while in action for Bristol in December, while Saracens hooker Campbell will have a knee operation next week.
Bristol lock Delaney Burns and Saracens prop Liz Crake have been added to the squad as cover.
Back row Alex Matthews has been ruled out of Saturday’s game against Scotland at Murrayfield because of a shoulder injury sustained in the 33-12 win against Ireland last Saturday.
